Clinical immunity
A new clustering method identifies multiple sclerosis-specific T-cell receptors
Ann Clin Transl Neurol. 2021 Jan;8(1):163-176.
Department of Neurology, Graduate School of Medical Sciences, Kyushu University
Fumie Hayashi
TCR repertoire analysis performed on the peripheral blood of patients with multiple sclerosis (MS) shows that the patient group is more diverse than the healthy group. Clustering analysis was also performed, and it was shown that TBV4-3 clusters are widely shared by patients with HLA-DRB1*04:05. Furthermore, it is predicted that the cluster group will recognize the CMV antigen.

Basal immunity
Skin T cells maintain their diversity and functionality in the elderly
Commun Biol. 2021 Jan 4;4(1):13.
Department of Dermatology, Course of Integrated Medicine, Graduate School of Medicine, Osaka University
Hanako Koguchi-Yoshioka
Our TCR repertoire analysis was used in a bilateral study of age-related changes in immune cells using Human resident memory T cells present in the skin and peripheral blood. It has been announced that the immune diversity of human resident memory T cells present in the skin of old people was maintained.

Clinical immunity
T‑cell receptor repertoire of cytomegalovirus‑specific cytotoxic T‑cells after allogeneic stem cell transplantation
Sci Rep. 2020 Dec 17;10(1):22218.
Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital
Takashi Toya
Our TCR repertoire analysis was used in the study of cytomegalovirus infection in allogeneic hematopoietic stem cell transplantation. The authors extracted cytomegalovirus-positive cells from the peripheral blood of 16 post-transplant patients and then searched for common clones between patients in the analysis of cytomegalovirus-specific T cells. Through this study, the authors suggest that it is possible to clarify how immune cells respond to cytomegalovirus reactivation after transplantation.

Clinical immunity
Aberrant mucosal immunoreaction to tonsillar microbiota in immunoglobulin A nephropathy
Nephrol Dial Transplant. 2021 Jan 1;36(1):75-86.
Division of Clinical Nephrology and Rheumatology, Kidney Research Center, Niigata University Graduate School of Medical and Dental Sciences
Hiroki Yamaguchi
Our BCR repertoire analysis was used in an immune response study in IgA nephropathy, an autoimmune disease. Differences in the immune cell repertoire between IgA nephropathy and tonsillitis patients were investigated.

Solid tumor
Adoptive transfer of zoledronateexpanded autologous Vγ9Vδ2 T-cells in patients with treatment-refractory nonsmall-cell lung cancer: a multicenter,open-label, single-arm, phase 2 study
Journal for ImmunoTherapy of Cancer 2020;8:e001185.
Department of Immunotherapeutics, The University of Tokyo Hospital
Kazuhiro Kakimi
In the report of clinical trials of non-small cell lung cancer using gamma delta T-cell therapy, our TCR repertoire analysis technology was used to monitor changes in the abundance frequency of major clones of delta T cells before and after treatment administration.

Basal immunity
Fibroblasts as a source of self-antigens for central immune tolerance
Nat Immunol. 2020 Oct;21(10):1172-1180.
Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo
Takeshi Nitta
Our TCR repertoire analysis was used in a study to explore the PDGFR⁺ gp38⁺ DPP4^- thymic fibroblast subset required to induce T-cell tolerance. Mouse TCR repertoire analysis shows that the selection of some TCR clones can be avoided by deleting a specific LTβR.

Clinical immunity
A Novel Synthetic Dual Agonistic Liposomal TLR4/7 Adjuvant Promotes Broad Immune Responses in an Influenza Vaccine With Minimal Reactogenicity
Front Immunol. 2020 Jun 19;11:1207.
Moores Cancer Center, University of California San Diego (UCSD)
Fumi Sato-Kaneko
Our BCR repertoire analysis was used in the study of a novel liposome preparation (TLR4/7 dual agonist) developed by UCSD. BCR repertoire analysis showed that this product can induce various B cells in the lymph nodes and induce antibodies against a wide range of hemagglutinin (HA) epitopes of the influenza virus. Based on these results, the development of a vaccine with high safety and a wide range of protective capabilities is expected by using the TLR4/7 dual liposome adjuvant.