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TCR/BCR Repertoire Analysis

Diversity analysis of immune cells involved in specific antigen recognition will be performed at the gene level. High quality repertoire analysis is being implemented by optimized methods such as unbiased amplification, next generation sequencing, and dedicated bioinformatics.

Neoepitope Analysis

By conducting RNA seq and Exose seq together, specific gene mutations which are specific to individual cancers can be identified. In addition, by taking into account intermolecular coupling, variant peptides (neoepitope) capable of presenting an antigen will be identified. This identification can also be used for indel, long indel, and splicing variants using sequence data.

16S rRNA Bacterial Flora Analysis

Distribution of bacterial species (bacterial flora) in samples can be analyzed by examining the V1V2 or V3V4 region of the 16S rRNA gene preserved in each bacterial species using next generation sequencing. This analysis can be applied not only to biologically derived samples (feces, saliva, etc.), but also to environmentally derived samples (soil, food, etc.).



JCI Insight

Clinical immunity

Dr. Masayo Ukita in the Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine has published a paper in "JCI Insight" titled "CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer".


Scientific Reports

Dr. Koichiro Yoshimaru in the Department of Pediatric Surgery, Kyushu University Graduate School of Medical Sciences has published a paper in "Scientific Reports" titled "Dental pulp stem cells as a therapy for congenital entero-neuropathy".


Nature Communications

Basal immunity

Dr. Yasuhiro Kazuki in the Chromosome Engineering Research Center, Tottori University has published in "Nature Communications" titled "Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci".

TCR/BCR Repertoire analysis Guidebook

MUST READ! For users considering Repertoire analysis

An easy-to-understand repertoire analysis guidebook has been prepared which explains in plain language for the questions such as what is immune repertoire analysis and what kind of preparation is necessary.
Please request the guidebook from below if desired.

Get GuideBook

Get GuideBook

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Number of pages:45, File size: 8.4 MB, File format: PDF

<Table of Contents>


Chapter 1: TCR/BCR Repertoire Analysis

  • Somatic recombination and hypermutation
  • Scale of diversity
  • Differences in gene structure between gDNA and mRNA after somatic recombination

Chapter 2:
Analysis of Repertoire

  • History of repertoire analysis and our story
  • Gene amplification method
  • Risks of PCR bias
  • Risk of primer mismatch in Multiplex PCR
  • Inability of Multiplex PCR to determine the immunoglobulin isotype
  • "Pair Identification" of repertoire using single cell analysis

Chapter 3:
Specimen Handling

  • Processing and shipping method
  • An example of shipping a sample to our company (dry ice packaging)
  • RNA stabilization of blood (whole blood), PBMC,
  • cultured cell or sorted cell and tissues and total
  • RNA samples

Chapter 4:
Interpretation of Analysis Data

  • 2D and 3D graph, Read Ranking
  • Diversity index
  • Comparison between samples
  • FAQ

Chapter 5:

  • Basal immunity <13 reports>
  • Clinical immunity <11 reports>
  • Hematological malignancy <6 reports>
  • Tumor immunity <8 reports>

Customer Feedback

University professor A

The realization of personalized medicine requires the identification of tumor antigens and cytotoxic T cell therapy to target tumor antigens. Neoepitope search・identification software and TCR repertoire analysis provided by Repertoire Genesis Inc. made it possible to identify targets and corresponding TCR for subsequent application to gene therapy, enabling dendritic cell vaccination therapy. One can truly feel that this marks the beginning of cutting-edge medicine.

University professor B
Field:Hematological oncology

By TCR/BCR repertoire analysis, various clinical issues can be solved. TCR/BCR repertoire analysis is considered necessary for evaluating and predicting the success or failure of bone marrow transplantation in our lab; thus, we request repertoire analysis frequently. Moreover, this analysis is highly useful for clinical diagnosis and determination of treatment efficacy. Neoepitope analysis recently developed by Repertoire Genesis Inc. can be applied widely in the hematology field and it is believed that the analysis can be quickly used for predicting therapy effect for CML. It is a technology progressively increasing clinical knowledge with extremely broad scope of application.

University professor C
Field:Hematological oncology

Our lab is carrying out studies of infectious disease, allergy, and autoimmune disease based on hematology and immunology, pursuing diagnostic methods which can be applied across organs. Within our research, determining whether the immune system is specifically involved in disease using TCR/BCR repertoire analysis developed by Repertoire Genesis, Inc. plays an extremely important role. Moreover, we think that the cause of idiopathic purpura will be elucidated by TCR/BCR repertoire analysis.

Director of a research institution D

By TCR/BCR repertoire analysis, the research field of infection immunity is thought to progress dramatically. Until recently, the importance of T cell involvement has been elucidated using a virus infected animal model. In the future, we expect to apply BCR analysis for developing antibody therapy drugs. Thus, continuously analyses are requested to Repertoire Genesis, Inc.

University professor E

To study the relationship between lifestyle diseases (obesity) and the immune system, we are using an arteriosclerosis animal model for analysis. We consider TCR/BCR repertoire analysis to be the most effective tool for conducting precise characterization analysis of cells infiltrating the arteriosclerotic region.
Analysis results by Repertoire Genesis Inc. showed that involvement of NKT cells in lifestyle diseases is strongly indicated. Research from a perspective of relationship between lifestyle diseases (obesity) and the immune system may seem weak at first. However, the relationship between many diseases and the immune system will be elucidated by technologies provided by Repertoire Genesis Inc. Thus, progress in future research is anticipated.

University professor F
Field:Hematological oncology

The fatality rate of ATL found in Kyushu area is extremely high after onset. In recent years, success in treating ATL by opdivo has been confirmed. However, the mode of action for drug efficacy is not clear.
TCR repertoire analysis by Repertoire Genesis Inc. revealed the involvement between the hemogram pattern in peripheral blood and Treg after administration of opdivo. Therefore, the analysis results may bring some good news in the future. Moreover, it appears that a final confirmation of ATL metastasis to the skin will be performed by TCR repertoire. It is also thought that many cases of leukemia and lymphoma will require TCR/BCR repertoire analysis. We intend to continue using the service provided by Repertoire Genesis Inc.

University professor G (overseas)

Now we would have extreme use of Repertoire Genesis's sequencing technology because sequencing T cells on a massive scale would provide more useful representation of the TCR repertoire and provide a fundamental basis for progressing to more detailed cell culture/in vivo experimentation.

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