The realization of personalized medicine requires the identification of tumor antigens and cytotoxic T cell therapy to target tumor antigens. Neoepitope search・identification software and TCR repertoire analysis provided by Repertoire Genesis Inc. made it possible to identify targets and corresponding TCR for subsequent application to gene therapy, enabling dendritic cell vaccination therapy. One can truly feel that this marks the beginning of cutting-edge medicine.

By TCR/BCR repertoire analysis, various clinical issues can be solved. TCR/BCR repertoire analysis is considered necessary for evaluating and predicting the success or failure of bone marrow transplantation in our lab; thus, we request repertoire analysis frequently. Moreover, this analysis is highly useful for clinical diagnosis and determination of treatment efficacy. Neoepitope analysis recently developed by Repertoire Genesis Inc. can be applied widely in the hematology field and it is believed that the analysis can be quickly used for predicting therapy effect for CML. It is a technology progressively increasing clinical knowledge with extremely broad scope of application.

Our lab is carrying out studies of infectious disease, allergy, and autoimmune disease based on hematology and immunology, pursuing diagnostic methods which can be applied across organs. Within our research, determining whether the immune system is specifically involved in disease using TCR/BCR repertoire analysis developed by Repertoire Genesis, Inc. plays an extremely important role. Moreover, we think that the cause of idiopathic purpura will be elucidated by TCR/BCR repertoire analysis.

By TCR/BCR repertoire analysis, the research field of infection immunity is thought to progress dramatically. Until recently, the importance of T cell involvement has been elucidated using a virus infected animal model. In the future, we expect to apply BCR analysis for developing antibody therapy drugs. Thus, continuously analyses are requested to Repertoire Genesis, Inc.

To study the relationship between lifestyle diseases (obesity) and the immune system, we are using an arteriosclerosis animal model for analysis. We consider TCR/BCR repertoire analysis to be the most effective tool for conducting precise characterization analysis of cells infiltrating the arteriosclerotic region.
Analysis results by Repertoire Genesis Inc. showed that involvement of NKT cells in lifestyle diseases is strongly indicated. Research from a perspective of relationship between lifestyle diseases (obesity) and the immune system may seem weak at first. However, the relationship between many diseases and the immune system will be elucidated by technologies provided by Repertoire Genesis Inc. Thus, progress in future research is anticipated.

The fatality rate of ATL found in Kyushu area is extremely high after onset. In recent years, success in treating ATL by opdivo has been confirmed. However, the mode of action for drug efficacy is not clear.
TCR repertoire analysis by Repertoire Genesis Inc. revealed the involvement between the hemogram pattern in peripheral blood and Treg after administration of opdivo. Therefore, the analysis results may bring some good news in the future. Moreover, it appears that a final confirmation of ATL metastasis to the skin will be performed by TCR repertoire. It is also thought that many cases of leukemia and lymphoma will require TCR/BCR repertoire analysis. We intend to continue using the service provided by Repertoire Genesis Inc.

Now we would have extreme use of Repertoire Genesis? s sequencing technology because sequencing T cells on a massive scale would provide more useful representation of the TCR repertoire and provide a fundamental basis for progressing to more detailed cell culture/in vivo experimentation.