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TCR/BCR Repertoire Analysis
Diversity analysis of immune cells involved in specific antigen recognition will be performed at the gene level. High quality repertoire analysis is being implemented by optimized methods such as unbiased amplification, next generation sequencing, and dedicated bioinformatics.
Neoepitope Analysis
By conducting RNA seq and Exose seq together, specific gene mutations which are specific to individual cancers can be identified. In addition, by taking into account intermolecular coupling, variant peptides (neoepitope) capable of presenting an antigen will be identified. This identification can also be used for indel, long indel, and splicing variants using sequence data.
16S rRNA Bacterial Flora Analysis
Distribution of bacterial species (bacterial flora) in samples can be analyzed by examining the V1V2 or V3V4 region of the 16S rRNA gene preserved in each bacterial species using next generation sequencing. This analysis can be applied not only to biologically derived samples (feces, saliva, etc.), but also to environmentally derived samples (soil, food, etc.).
Publications
- 2024/09/12
- Paper
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Cancer Research Communications
Solid tumor
Therapeutic efficacy of IL-7/CCL19-expressing CAR-T cells in intractable solid tumor models of glioblastoma and pancreatic cancerA paper utilizing our TCR Repertoire Analysis was published by Dr. Keisuke Ota, Department of Immunology, Yamaguchi University Graduate School of Medicine, who has investigated the therapeutic efficacy of next-generation CAR-T cells (7×19 CAR-T) that produce IL-7 and CCL19 against glioblastoma and pancreatic cancer, which are refractory cancers, using mouse models.
To confirm the cytotoxic activity and therapeutic efficacy of 7×19 CAR-T, they experimented two models using anti-EGFRvIII CAR-T generated from epidermal growth factor receptor variant III (EGFRvIII)-positive glioblastoma and healthy donor PBMCs, and human epidermal growth factor receptor 2 (HER2)-positive pancreatic cancer organoids and in vitro and in vivo evaluation in models with anti-HER2 CAR-Ts generated from PBMCs of the same patients. The results by each experiment were induced the result in prolonged survival in mice. This study is the first to demonstrate the therapeutic efficacy of next-generation CAR-T in an autologous model using patient-derived tumor organoid and CAR-T generated from the same patient's PBMC, in which unwanted allogeneic immune responses are fully excluded.
We have performed a Repertoire Analysis using TCR α and β chains before (from PBMC creation) and after (from splenocytes) injection of 7×19 CAR-Ts sorted CAR-positive and negative T cells.
- 2024/08/15
- Paper
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Clinical immunity
Analysis of B-cell receptor repertoire to evaluate immunogenicity of monovalent Omicron XBB.1.5 mRNA vaccinesA paper utilizing our Repertoire Analysis was published by Dr. Yohei Funakoshi and Dr. Kimikazu Yakushijin, the Department of Medicine, Division of Medical Oncology/Hematology, Kobe University Hospital and Graduate School of Medicine, and Dr. Goh Ohji, the Department of Microbiology and Infectious Diseases, Division of Infection Disease Therapeutics, Kobe University Hospital.
In this paper, they evaluated the Omicron XBB.1.5 mRNA vaccine using a new novel coronavirus-specific antibody sequence quantification method developed in the laboratory (QASAS method), and the results suggest that antibody production against the Omicron XBB strain is stronger than that of previous vaccines.
Our BCR Repertoire Analysis is used to obtain antibody gene sequences for matching against the novel coronavirus-specific antibody database.
- 2024/07/22
- Paper
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Involvement of the genus Corynebacterium in the pathogenesis of pigmented intratarsal keratinous cyst
A paper utilizing our 16S rRNA Bacterial Flora Analysis was published by Dr. Miyuki Yoshikawa, Dr. Daiki Rokunohe et al., Hirosaki University Graduate School of Medicine, Department of Dermatology, has published a paper on inflammation and bacterial flora in intratarsal keratinous cysts (IKC), which are pigmented intraocular keratinocysts.
IKC, a benign cystic lesion of the eyelid, usually appears as a yellow to white cyst, but rarely as a brown or gray-blue cyst, affecting the clinical diagnosis. The cause of this phenomenon is not known. The author's research group has observed a localized lymphocytic infiltrate in the melanocyte-rich, melanin-positive area below the cyst wall in pigmented IKC, and has analyzed the bacterial colonies within the cyst for the presence of Corynebacterium species. The etiology of pigmented IKC related to inflammation and bacterial flora was discussed in the paper.
We have performed 16S rRNA Bacterial Flora Analysis using the bacterial colonies in the cysts described in the paper.
TCR/BCR Repertoire analysis Guidebook
MUST READ! For users considering Repertoire analysis
An easy-to-understand repertoire analysis guidebook has been prepared which explains in plain language for the questions such as what is immune repertoire analysis and what kind of preparation is necessary.
Please request the guidebook from below if desired.
