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  • Researchers of Akita University used our BCR repertoire analysis and their paper has published in "Scientific Reports".

Researchers of Akita University used our BCR repertoire analysis and their paper has published in "Scientific Reports".

2019/06/20

A research work of Prof. Makoto Hirokawa in Akita University Graduate School of Medicine Department of General Internal Medicine and Clinical Laboratory Medicine has published in "Scientific Reports" titled "High-throughput sequencing of IgG B-cell receptors reveals frequent usage of the rearranged IGHV4-28/IGHJ4 gene in primary immune thrombocytopenia".

This study is related to Primary immune thrombocytopenia(ITP), which aimed to clarify the immune response. Our BCR repertoire analysis was used to measure B cell receptor (BCR) IgG.
As a result of measurement, in the patient group, IGHV4-28 was often used in the V gene region of IgG. Most of this used IGHJ4 segment in the J gene region. There were also multiple related clones with single amino substitution in the CDR3 region suggesting somatic hypermutation.
The increase in B cells that express IgG containing these IGHV4-28 / IGHJ4 may be due to specific antigens. And Our analysis suggests that it may help to elucidate the immune pathophysiology of ITP.